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superscript rt ii kit random hexamers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript rt ii kit random hexamers
    Superscript Rt Ii Kit Random Hexamers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rt+ii+kit+random+hexamers/pmc11695996-320-50-51
    Average 90 stars, based on 1 article reviews
    superscript rt ii kit random hexamers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: A key role for p60-Katanin in axon navigation is conditioned by the tubulin polyglutamylase TTLL6
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, Illkirch, France) from 200 ng of katna1 +/+ and katna1 -/-MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5’-ATGTGGAGCACAGATCGTCTCCATGTG-3’ Katna1RTPCRex5-6_REV: 5’-CAGCAATGTCATCCCATGTGACATTGGG-3’. .. For quantitative RT-PCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 -/-MZ and one pool of 10 wild-type embryos (24 hpf) using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, Courtaboeuf, France) using the primers listed in . ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes.
    Article Snippet: The microtubule cytoskeleton is a major driving force of neuronal circuit development.. Fine-tuned remodelling of this network by selective activation of microtubule-regulating proteins, including microtubule-severing enzymes, has emerged as a central process in neuronal wiring.. Tubulin posttranslational modifications control both microtubule properties and the activities of their interacting proteins.

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1 +/+ and katna1 −/−MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5′-ATGTGGAGCACAGATCGTCTCCATGTG-3′ Katna1RTPCRex5-6_REV: 5′-CAGCAATGTCATCCCATGTGACATTGGG-3′. .. In RT-qPCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 −/−MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, France) using the primers listed in Table . ..

    Gene Expression:

    Article Title: A key role for p60-Katanin in axon navigation is conditioned by the tubulin polyglutamylase TTLL6
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, Illkirch, France) from 200 ng of katna1 +/+ and katna1 -/-MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5’-ATGTGGAGCACAGATCGTCTCCATGTG-3’ Katna1RTPCRex5-6_REV: 5’-CAGCAATGTCATCCCATGTGACATTGGG-3’. .. For quantitative RT-PCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 -/-MZ and one pool of 10 wild-type embryos (24 hpf) using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, Courtaboeuf, France) using the primers listed in . ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes.
    Article Snippet: The microtubule cytoskeleton is a major driving force of neuronal circuit development.. Fine-tuned remodelling of this network by selective activation of microtubule-regulating proteins, including microtubule-severing enzymes, has emerged as a central process in neuronal wiring.. Tubulin posttranslational modifications control both microtubule properties and the activities of their interacting proteins.

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1 +/+ and katna1 −/−MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5′-ATGTGGAGCACAGATCGTCTCCATGTG-3′ Katna1RTPCRex5-6_REV: 5′-CAGCAATGTCATCCCATGTGACATTGGG-3′. .. In RT-qPCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 −/−MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, France) using the primers listed in Table . ..

    Reverse Transcription:

    Article Title: A key role for p60-Katanin in axon navigation is conditioned by the tubulin polyglutamylase TTLL6
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, Illkirch, France) from 200 ng of katna1 +/+ and katna1 -/-MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5’-ATGTGGAGCACAGATCGTCTCCATGTG-3’ Katna1RTPCRex5-6_REV: 5’-CAGCAATGTCATCCCATGTGACATTGGG-3’. .. For quantitative RT-PCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 -/-MZ and one pool of 10 wild-type embryos (24 hpf) using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, Courtaboeuf, France) using the primers listed in . ..

    Article Title: Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Article Snippet: .. Total RNA from adult pancreas was isolated using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (Eurobiogreen QPCR Mix, Hi-ROX; Eurobio, Courtaboeuf, France). ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1 +/+ and katna1 −/−MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5′-ATGTGGAGCACAGATCGTCTCCATGTG-3′ Katna1RTPCRex5-6_REV: 5′-CAGCAATGTCATCCCATGTGACATTGGG-3′. .. In RT-qPCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 −/−MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, France) using the primers listed in Table . ..

    Real-time Polymerase Chain Reaction:

    Article Title: A key role for p60-Katanin in axon navigation is conditioned by the tubulin polyglutamylase TTLL6
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, Illkirch, France) from 200 ng of katna1 +/+ and katna1 -/-MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5’-ATGTGGAGCACAGATCGTCTCCATGTG-3’ Katna1RTPCRex5-6_REV: 5’-CAGCAATGTCATCCCATGTGACATTGGG-3’. .. For quantitative RT-PCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 -/-MZ and one pool of 10 wild-type embryos (24 hpf) using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, Courtaboeuf, France) using the primers listed in . ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes.
    Article Snippet: The microtubule cytoskeleton is a major driving force of neuronal circuit development.. Fine-tuned remodelling of this network by selective activation of microtubule-regulating proteins, including microtubule-severing enzymes, has emerged as a central process in neuronal wiring.. Tubulin posttranslational modifications control both microtubule properties and the activities of their interacting proteins.

    Article Title: Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Article Snippet: .. Total RNA from adult pancreas was isolated using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (Eurobiogreen QPCR Mix, Hi-ROX; Eurobio, Courtaboeuf, France). ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1 +/+ and katna1 −/−MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5′-ATGTGGAGCACAGATCGTCTCCATGTG-3′ Katna1RTPCRex5-6_REV: 5′-CAGCAATGTCATCCCATGTGACATTGGG-3′. .. In RT-qPCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 −/−MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, France) using the primers listed in Table . ..

    SYBR Green Assay:

    Article Title: A key role for p60-Katanin in axon navigation is conditioned by the tubulin polyglutamylase TTLL6
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, Illkirch, France) from 200 ng of katna1 +/+ and katna1 -/-MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5’-ATGTGGAGCACAGATCGTCTCCATGTG-3’ Katna1RTPCRex5-6_REV: 5’-CAGCAATGTCATCCCATGTGACATTGGG-3’. .. For quantitative RT-PCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 -/-MZ and one pool of 10 wild-type embryos (24 hpf) using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, Courtaboeuf, France) using the primers listed in . ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes.
    Article Snippet: The microtubule cytoskeleton is a major driving force of neuronal circuit development.. Fine-tuned remodelling of this network by selective activation of microtubule-regulating proteins, including microtubule-severing enzymes, has emerged as a central process in neuronal wiring.. Tubulin posttranslational modifications control both microtubule properties and the activities of their interacting proteins.

    Article Title: Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Article Snippet: .. Total RNA from adult pancreas was isolated using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (Eurobiogreen QPCR Mix, Hi-ROX; Eurobio, Courtaboeuf, France). ..

    Article Title: Tubulin glutamylation regulates axon guidance via the selective tuning of microtubule-severing enzymes
    Article Snippet: Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScriptTM III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1 +/+ and katna1 −/−MZ RNA extracts using the following primers: Katna1RTPCRex3-4_FOR: 5′-ATGTGGAGCACAGATCGTCTCCATGTG-3′ Katna1RTPCRex5-6_REV: 5′-CAGCAATGTCATCCCATGTGACATTGGG-3′. .. In RT-qPCR analysis of katna1 homologous gene expression levels, RNAs were extracted from 5 independent pools of 10 katna1 +/+ , 10 katna1 −/−MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix, Hi-ROX; Eurobio Scientific, France) using the primers listed in Table . ..

    Isolation:

    Article Title: Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Article Snippet: .. Total RNA from adult pancreas was isolated using the RNeasy Mini-kit (Qiagen, Courtaboeuf, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, Illkirch, France). qPCR was performed using a SYBR Green master mix (Eurobiogreen QPCR Mix, Hi-ROX; Eurobio, Courtaboeuf, France). ..



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    Thermo Fisher superscript rt ii kit random hexamers
    Superscript Rt Ii Kit Random Hexamers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rt+ii+kit+random+hexamers/pmc11695996-320-50-51
    Average 90 stars, based on 1 article reviews
    superscript rt ii kit random hexamers - by Bioz Stars, 2026-09
    90/100 stars
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